mouse monoclonal anti-cb2 antibody  (Abnova)

Journal: Frontiers in Endocrinology

Article Title: The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction in vivo

doi: 10.3389/fendo.2023.1269334

Figure Lengend Snippet: Primary and secondary antisera used for Western blot, IFL, and IHC.

Article Snippet: CB2 (3C7) mouse monoclonal IgG (sc-293188, Santa Cruz Biotechnology) , 1:3000.

Techniques: Western Blot, Plasmid Preparation

Journal: Frontiers in Endocrinology

Article Title: The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction in vivo

doi: 10.3389/fendo.2023.1269334

Figure Lengend Snippet: Distribution of CB1 and CB2 in rat testis treated with KP and AEA. Double immunofluorescence of CB1 (green) and CB2 (red) expression in control (C), kisspeptin (KP), anandamide (AEA), and SR141716A plus AEA (SR + AEA)-treated animals ( n = 4 for each group). Magnification = C and KP ×63; AEA and SR + AEA, ×40. * Elongated spermatids, ↑ spermatocytes, ^ spermatogonia, Δ Sertoli cells, ° interstitium.

Article Snippet: CB2 (3C7) mouse monoclonal IgG (sc-293188, Santa Cruz Biotechnology) , 1:3000.

Techniques: Immunofluorescence, Expressing, Control

Journal: Translational Lung Cancer Research

Article Title: Cannabinoid receptor 2 expression in early-stage non-small cell lung cancers identifies patients with good prognosis and longer survival

doi: 10.21037/tlcr-22-247

Figure Lengend Snippet: Patients’ characteristics categorized by gender, clinical stage, grading, and histology

Article Snippet: Samples were stained using mouse monoclonal anti-CB1 antibody (ImmunoGenes, Cat# 01, RRID:AB_2910137) and mouse monoclonal anti-CB2 antibody (Abnova, Cat# H00001269-M01, RRID:AB_875479) according to the manufacturer’s protocol.

Techniques: Paraffin-embedded Immunohistochemistry

Journal: Translational Lung Cancer Research

Article Title: Cannabinoid receptor 2 expression in early-stage non-small cell lung cancers identifies patients with good prognosis and longer survival

doi: 10.21037/tlcr-22-247

Figure Lengend Snippet: Kaplan-Meier analysis of OS, CSS, and DFS for CB2 gene (A) and protein (B) expression positivity in tumor tissue. CB2, cannabinoid receptor 2; qRT-PCR, quantitative reverse-transcriptase polymerase chain reaction; neg., negative; pos., positive; HR, hazard ratio; IHC, immunohistochemistry; OS, overall survival; CSS, cancer specific survival; DFS, disease free survival.

Article Snippet: Samples were stained using mouse monoclonal anti-CB1 antibody (ImmunoGenes, Cat# 01, RRID:AB_2910137) and mouse monoclonal anti-CB2 antibody (Abnova, Cat# H00001269-M01, RRID:AB_875479) according to the manufacturer’s protocol.

Techniques: Expressing, Quantitative RT-PCR, Reverse Transcription, Polymerase Chain Reaction, Immunohistochemistry

Journal: Translational Lung Cancer Research

Article Title: Cannabinoid receptor 2 expression in early-stage non-small cell lung cancers identifies patients with good prognosis and longer survival

doi: 10.21037/tlcr-22-247

Figure Lengend Snippet: Patients’ characteristics categorized by weight and BMI

Article Snippet: Samples were stained using mouse monoclonal anti-CB1 antibody (ImmunoGenes, Cat# 01, RRID:AB_2910137) and mouse monoclonal anti-CB2 antibody (Abnova, Cat# H00001269-M01, RRID:AB_875479) according to the manufacturer’s protocol.

Techniques: Paraffin-embedded Immunohistochemistry

Journal: Translational Lung Cancer Research

Article Title: Cannabinoid receptor 2 expression in early-stage non-small cell lung cancers identifies patients with good prognosis and longer survival

doi: 10.21037/tlcr-22-247

Figure Lengend Snippet: CB1 (A) and CB2 (B) gene expression (ΔCT values) in tumor and tumor-free lung tissue from 10 NSCLC patients. NSCLC, non-small cell lung cancer; CB1, cannabinoid receptor 1; CT, cycle threshold; CB2, cannabinoid receptor 2.

Article Snippet: Samples were stained using mouse monoclonal anti-CB1 antibody (ImmunoGenes, Cat# 01, RRID:AB_2910137) and mouse monoclonal anti-CB2 antibody (Abnova, Cat# H00001269-M01, RRID:AB_875479) according to the manufacturer’s protocol.

Techniques: Expressing

Journal: Translational Lung Cancer Research

Article Title: Cannabinoid receptor 2 expression in early-stage non-small cell lung cancers identifies patients with good prognosis and longer survival

doi: 10.21037/tlcr-22-247

Figure Lengend Snippet: Immunohistochemical staining of FFPE tumor tissue samples of NSCLC patients using anti-CB1 and anti-CB2 monoclonal antibodies (under ×10 lens). IHC, immunohistochemistry; CB1, cannabinoid receptor 1; CB2, cannabinoid receptor 2; FFPE, formalin-fixed paraffin-embedded; NSCLC, non-small cell lung cancer.

Article Snippet: Samples were stained using mouse monoclonal anti-CB1 antibody (ImmunoGenes, Cat# 01, RRID:AB_2910137) and mouse monoclonal anti-CB2 antibody (Abnova, Cat# H00001269-M01, RRID:AB_875479) according to the manufacturer’s protocol.

Techniques: Immunohistochemical staining, Staining, Immunohistochemistry, Formalin-fixed Paraffin-Embedded

Journal: Molecules (Basel, Switzerland)

Article Title: Role of Caryophyllane Sesquiterpenes in the Entourage Effect of Felina 32 Hemp Inflorescence Phytocomplex in Triple Negative MDA-MB-468 Breast Cancer Cells.

doi: 10.3390/molecules26216688

Figure Lengend Snippet: Figure 8. Modulation of CB2 receptor expression in MDA-MB-468 cells detected after 24 h treatment with 10 µg/mL FOJ (organic extract from Felina 32 hemp inflorescences collected in June), the corresponding mFOJ cocktail, and with the nonintoxicating phytocannabinoids and caryophyllane sesquiterpenes contained in FOJ extract. (A) Representative images obtained at immunofluorescence analysis. (B) Densitometric analysis of immunofluorescence carried out by the free software ImageJ (n = 6). ** p < 0.01 and *** p < 0.001, significant difference respect to the control (ANOVA + Dunnett’s Multiple Comparison PostTest). 1. Control; 2. FOJ; 3. mFOJ; 4. Cannabidiol, 0.8 µg/mL; 5. Cannabichromene, 0.04 µg/mL; 6. α-Humulene, 0.01 µg/mL; 7. β-Caryophyllene, 0.01 µg/mL; 8. β-Caryophyllene oxide, 0.007 µg/mL.

Article Snippet: After blocking with 3% bovine serum albumin (BSA) in PBS for 30 min at room temperature, cells were incubated for 1 h, at room temperature, with Molecules 2021, 26, 6688 18 of 20 mouse monoclonal anti-CB2 antibody 1:150 (Santa Cruz Biotechnology, Inc., Dallas, TE, USA).

Techniques: Expressing, Software, Control, Comparison

Journal: Molecules (Basel, Switzerland)

Article Title: Role of Caryophyllane Sesquiterpenes in the Entourage Effect of Felina 32 Hemp Inflorescence Phytocomplex in Triple Negative MDA-MB-468 Breast Cancer Cells.

doi: 10.3390/molecules26216688

Figure Lengend Snippet: Figure 9. Modulation of CB2 receptor expression in MDA-MB-468 cells detected after 24 h treatment with 10 µg/mL FOS (organic extract from Felina 32 hemp inflorescences collected in September), the corresponding mFOS cocktail, and with the nonintoxicating phytocannabinoids and caryophyllane sesquiterpenes contained in FOS extract. (A) Representative images obtained at immunofluorescence analysis. (B) Densitometric analysis of immunofluorescence carried out by the free software ImageJ (n = 6). ** p < 0.01 and *** p < 0.001, significant difference respect to the control (ANOVA + Dunnett’s Multiple Comparison PostTest). 1. Control; 2. FOS; 3. mFOS; 4. Cannabidiol, 3.4 µg/mL; 5. Cannabichromene, 1.2 µg/mL; 6. α-Humulene, 0.02 µg/mL; 7. β-Caryophyllene, 0.02 µg/mL; 8. β-Caryophyllene oxide, 0.02 µg/mL.

Article Snippet: After blocking with 3% bovine serum albumin (BSA) in PBS for 30 min at room temperature, cells were incubated for 1 h, at room temperature, with Molecules 2021, 26, 6688 18 of 20 mouse monoclonal anti-CB2 antibody 1:150 (Santa Cruz Biotechnology, Inc., Dallas, TE, USA).

Techniques: Expressing, Software, Control, Comparison